IMP-1 encoded by a novel Tn402-like class 1 integron in clinical Achromobacter xylosoxidans, China

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IMP-1 encoded by a novel Tn402-like class 1 integron in clinical Achromobacter xylosoxidans, China

Achromobacter xylosoxidans strain A22732 is isolated from a pneumonia patient in China and produces carbapenemases OXA-114e and IMP-1, which are encoded by chromosome and plasmid, respectively, and confer resistance to multiple ß-lactam antibiotics including carbapenems. The blaIMP-1 gene together with aacA7 and orfE is captured by a novel Tn402-like class 1 integron in a conjugative IncP-1ß pl...

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Preclinical class 1 integron with a complete Tn402-like transposition module.

The presence of integrons was assessed in gut bacteria isolated from wild-caught prawns. A pseudomonad was recovered that contained a Tn402-like class 1 integron with a complete transposition module and two gene cassettes. One cassette was identical to a previously described cassette from a chromosomal class 3 integron in Delftia tsuruhatensis.

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Detection of gene cassettes in Tn402-like class 1 integrons.

Amplification of the gene cassettes in class 1 integrons by PCR using primers in the 5 conserved segment (5 -CS) and the 3 -CS (7, 8) has been used in hundreds of studies to identify integron-associated resistance genes (Fig. 1A). Equivalent PCR primers that detect cassettes in class 2 integrons, i.e., Tn7 family transposons (18), are also widely used. The approach used to detect integrons in a...

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First report of a Tn402-like class 1 integron carrying blaVIM-2 in Pseudomonas putida from Argentina.

1 Departamento de Microbiología, Instituto de Biología Molecular y Celular de Rosario (IBR, CONICET), Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, 2 Departamento de Química Biológica, Instituto de Biología Molecular y Celular de Rosario (IBR, CONICET), Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, 3 Servicio de Bacteriol...

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Osteomyelitis caused by Achromobacter xylosoxidans

Achromobacter xylosoxidans is an aerobic, nonfermenting gram-negative rod and described as a waterborne bacterium since it habits aquatic environments ubiquitously. It has frequently been isolated from aquatic surroundings in the hospital and from various human body sites. Although occasionally considered a non-pathogen, A. xylosoxidans has been associated with outbreaks of nosocomial infection...

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ژورنال

عنوان ژورنال: Scientific Reports

سال: 2014

ISSN: 2045-2322

DOI: 10.1038/srep07212